LGC·SeraCare·KPL (hereinafter referred to as KPL), distributed by XMJ, is a world-renowned manufacturer of commercially available affinity-purified secondary antibodies and substrate chromogenic systems. With over 40 years of product R&D experience, KPL has obtained ISO 13485 quality management system certification. Its products feature consistent batch-to-batch performance and reliable quality, earning wide recognition and praise from diagnostic companies worldwide.

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KPL’s product portfolio includes cost-effective secondary antibodies, substrate chromogenic reagents, and immunoassay auxiliary reagents, which are widely used in immunological research, diagnostic reagent development, and clinical testing. This article will focus on KPL’s high-cost-effectiveness ELISA substrate product series.

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In ELISA detection, 3,3',5,5'-tetramethylbenzidine (TMB) has become the preferred substrate for horseradish peroxidase (HRP) chromogenic detection, thanks to its high sensitivity, strong absorbance, ease of operation, safety, and broad compatibility.

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SureBlue? TMB, developed by KPL, is an optimized version of conventional TMB substrates. With over 30 years of application history, it offers higher sensitivity, consistent quality, low batch-to-batch variation, and non-carcinogenic properties. SureBlue Reserve? TMB is a further optimized iteration of SureBlue? TMB. Its detection sensitivity is 50% higher than that of SureBlue? TMB. As a water-based substrate (free of DMF or DMSO), it adopts a ready-to-use single-component formulation for convenient operation and has a shelf life of up to 24 months.

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ABTS? is a universal substrate containing 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). It offers a broad working range, making it suitable for scenarios requiring low background and moderate sensitivity.In the presence of horseradish peroxidase (HRP)-labeled conjugates, ABTS develops a blue-green color at 405–410 nm, and the color remains stable after the reaction is stopped. It is recommended for ELISA applications and is available in both single-component and two-component formats.

BluePhos? is a substrate specifically designed for alkaline phosphatase (AP)-labeled conjugates and serves as a high-performance alternative to disodium 4-nitrophenyl phosphate (pNPP). As a soluble, patented form of BCIP, it offers higher sensitivity, a broader working range, lower background signals, and greater ease of use. Supplied in a two-component format, this substrate is ready to use once mixed. It produces a blue product when reacted in microplates or test tubes, making it suitable for both qualitative and quantitative immunoassays. Compared to orange or yellow substrates, it provides clearer visual differentiation.

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LumiGLO? and LumiGLO Reserve? chemiluminescent substrates are luminol-based substrates specifically designed for use with horseradish peroxidase (HRP)-labeled reporters. LumiGLO Reserve? features enhanced signal intensity, with a sensitivity that is?over 20 times higher?than that of KPL LumiGLO? and other competing products. Both LumiGLO? and LumiGLO Reserve? chemiluminescent substrates are specialized for detecting low-abundance proteins or proteins derived from precious samples.

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Both PhosphaGLO? AP and PhosphaGLO Reserve? AP substrates are designed based on the dioxetane chemiluminescence principle, specifically for use with alkaline phosphatase (AP)-labeled reporters.Among them, PhosphaGLO Reserve? AP substrate delivers a significant boost in signal intensity, making it particularly suitable for detecting low-abundance proteins and target proteins in precious samples.The PhosphaGLO? and PhosphaGLO Reserve? AP substrate series are supplied as stable, single-component solutions. This format simplifies experimental operation while ensuring the stability and reliability of experimental results. Experimental results can be recorded using X-ray film or a chemiluminescence imager to obtain permanent records. For ELISA experiments, it is recommended to use a chemiluminometer for detection to achieve higher sensitivity and accuracy.

KPL’s substrate product series is highly favored by researchers and diagnostic reagent manufacturers for its exceptional performance. These substrates boast the following key features:

·?High sensitivity

·?Consistent batch-to-batch performance

·?Long shelf life

·?Flexible format options (single-component or multi-component)

·?Easy operation

·?Safety, environmental friendliness, and non-carcinogenicity

·?Multiple specifications to meet diverse needs

In addition to substrates, KPL also offers a full range of auxiliary reagents for experiments including enzyme-linked immunosorbent assay (ELISA), Western blotting (WB), immunohistochemistry (IHC), and enzyme-linked immunospot (ELISpot). These include coating buffers, blocking buffers, washing buffers, and stop solutions.

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For more information, please feel free to:

·?Call XMJ’s customer service hotline:?400-050-4006

·?Visit the official website:?www.mb020.com.cn

With its diverse product options and outstanding performance, KPL’s substrate series has become the?preferred brand?in the fields of immunological research and diagnostic reagent development.

KPL (Kinetic Plasmonics Laboratories) is a company boasting a profound history and an excellent reputation in the biosciences field. As one of the world’s early biotech firms to commercialize affinity-purified secondary antibodies, KPL has laid a solid foundation for its development by virtue of its pioneering status in this domain. Furthermore, KPL is also among the world’s major manufacturers of secondary antibodies and substrate chromogenic systems. With nearly 40 years of experience in product R&D, it has accumulated extensive professional knowledge and strong technical capabilities. The company has obtained ISO 13485 quality management system certification, which ensures minimal batch-to-batch variation and reliable quality for all its products. Characterized by high purity, high sensitivity, and a high signal-to-noise ratio (strong signal, low background), KPL’s products have won the trust of researchers and diagnostic enterprises worldwide. In 2013, KPL was acquired by SeraCare, a supplier of in vitro diagnostic (IVD) reagents, further consolidating its leading position in the industry. Founded in 1984, SeraCare is a key partner for global IVD manufacturers and clinical laboratories, and it became part of the LGC Group in 2018.

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As the exclusive distributor of the LGC·SeraCare·KPL brand in China, Beijing XMJ is committed to promoting KPL’s high-quality products in the Chinese market and providing premium products and professional services to a large number of domestic researchers.